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Plant |
#50588 |
pHSN401 |
CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance |
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#63142 |
pRGEB32 |
(Empty Backbone) Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation. |
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#51295 |
pRGEB31 |
(Empty Backbone) Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation. |
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Insect |
#49330 |
pAc-sgRNA-Cas9 |
Expresses sgRNA and Cas9-Puro in Drosophila S2 cells |
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C.elegans |
#47549 |
pDD162 |
Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome. |
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Yeast |
#43803 |
P426 |
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#43804 |
p415 |
Human Optimized S. pyogenes Cas9 |
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Bateria |
#42875 |
pCRISPR |
A crRNA expression plasmid for targeting a specific sequence |
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#84379 |
pRH2502 |
Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter |
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#84380 |
pRH2521 |
Expression of sgRNA from a imyc promoter (Pimyc) |
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#61737 |
pCRISPomyces-2 |
Streptomyces expression of codon-optimized Cas9 and custom gRNA |
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#42876 |
pCas9 |
Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide |
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Zebrafish |
#47929 |
pCS2-nCas9n |
expression of an optimized Cas9 for genome-editing in zebrafish |
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Mammalian |
#52970 |
FokI-dCas9 |
Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells |
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#61591 |
PX601 |
A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. |
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#42229 |
PX260 |
This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA. |
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#42230 |
PX330 |
A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid. |
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#48138 |
PX458[GFP] |
Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA |
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#48139 |
PX459[Puromycin] |
Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0) |
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#48140 |
PX461 |
Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA |
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#48141 |
PX462[Cas9n,Puromycin] |
Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA |
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#50661 |
pCW-Cas9[Tet-on,Puromycin] |
Inducible lentiviral expression of SpCas9 |
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#52963 |
LentiGuide-puro-Vector |
Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. Lentiviral backbone. |
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#52962 |
lentiCas9-Blast |
Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone. |
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#69982 |
pY010 (pcDNA3.1-hAsCpf1) |
Expresses humanized AsCpf1 |
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#19319 |
pLJM1-EGFP |
(Empty Backbone) 3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin |
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#12259 |
pMD2.G |
VSV-G envelope expressing plasmid |
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#12260 |
Lentivirus package plasmids |
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#61427 |
lenti sgRNA(MS2)_zeo backbone |
(Empty Backbone) 3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences. |
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#61426 |
lenti MS2-P65-HSF1_Hygro |
lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) |
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#61425 |
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3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) |
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lenti dCAS-VP64_Blast |
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#71814 |
eSpCas9(1.1) |
Expresses high specificity SpCas9. Px330-like plasmid. |
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#47327 |
pET-28b-Cas9-His |
For in vitro expression and purification of Cas9 protein |
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#62934 |
pET-NLS-Cas9-6xHis |
Expression of NLS-Cas9-6xHis in bacterial cells |
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86840 |
pJH373 |
mamalian expression of AcrIIA2 |
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84750 |
Lenti-AsCpf1-Blast |
Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. |
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84752 |
Lenti_gRNA-Puro |
(Empty Backbone) Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter |
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79145 |
pCAG-eCas9-GFP-U6-gRNA |
All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells |
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84745 |
pY30 |
Expresses huAsCpf1-P2A-puro and crRNA guide |
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84743 |
pY094 |
Expresses huAsCpf1-T2A-GFP and crRNA guide |
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84741 |
pY026 |
Expresses huAsCpf1 and crRNA guide |
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60958 |
PX552 |
pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. |
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79152 |
pC003 – LshC2C2 locus into pACYC184 for spacer cloning |
LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning |
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79150 |
pC001 – huLshC2C2-MBP for bacterial expression |
Expresses human codon-optimized LshC2c2 for purification in E. coli |
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64324 |
pU6-(BbsI)_CBh-Cas9-T2A-mCherry |
Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide |
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64222 |
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6 |
Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A |
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64218 |
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B |
Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A |
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64323 |
pU6-(BbsI)_CBh-Cas9-T2A-BFP |
Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide |
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51023 |
pSLQ1658-dCas9-EGFP |
Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system) |
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64108 |
pHAGE-TO-dCas9-3XmCherry |
Expression of Sp dCas9-3XmCherry in mammalian cells |
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60957 |
PX551 |
pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter. |
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100806 |
BE4-Gam |
Expresses BE4-Gam in mammalian cells |
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100809 |
SaBE4-Gam |
Expresses SaBE4-Gam in mammalian cells |
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102917 |
pCMV-ABE7.8 |
expresses ABE7.8 in mammalian cells |
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102919 |
pCMV-ABE7.10 |
expresses ABE7.10 in mammalian cells |
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108382 |
xCas9(3.7)-ABE(7.10) |
Mammalian expression vector for xCas9(3.7)-ABE |
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132778 |
pU6-Sp-pegRNA-HEK3_CTT_ins |
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132777 |
pU6-pegRNA-GG-acceptor |
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132775 |
pCMV-PE2 |
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62988 |
pSpCas9(BB)-2A-Puro (PX459) V2.0 |
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125647 |
pCMV-ABEmaxAW |
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64108 |
pHAGE-TO-dCas9-3XmCherry |
Expression of Sp dCas9-3XmCherry in mammalian cells |
